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Makerbot Talk @ Ignite, Wednesday 14/April

Title says it all. For those so inclined, here’s my very late notice that I’ll be talking about Makerbots, Repraps and Thingiverse this Wednesday 14th in the Science Museum. The event starts at 6:30. Ignite is an event that asks “Enlighten us, but make it quick”. It was started by Bre Pettis and Brady Forrest as a way for local communities to share ideas and raise the “collective IQ”, and is traditionally composed of a little Make contest first, (like “Best Bridge Made of Popsicle Sticks”) followed by a series of five minute talks.

Isolation and Culture of Bioluminescent Bacteria

Update: Keep your bacteria in the dark. I had been doing this without meaning to, and was confused to hear a fellow microbiologist growing a derivative of my cultures in her lab and getting no glow. When she grew them in the dark, they glowed again! Brief exposures have no effect. It’s the prevailing light conditions that seem to decide whether to stay active. This may be due to the light breaking down the little peptide the bacteria use to detect whether there are enough of them to glow meaningfully, or it might be a deliberate evolutionary adaptation.

The Makerbot Better Live Mousetrap Challenge

So, last night (being about 26 hours ago I think) I posted a challenge to the Makerbot User’s Group, asking for a live mousetrap that I could print, use, and award €25 for. Team Makerbot blogged it, and threw in a teeshirt if the winner was released under an open license, which just makes it all the more cool! Today, I received 5kg of plastic from Reprapsource.com, so I’m ready to start printing tomorrow.

Homebrew Bacterial Media for the Microbiology Hobbyist or DIYbio Enthusiast

Update: The links to these files have always been precarious, sorry.. I’ve just re-uploaded the two main files, and soon I’ll be reworking them into my “biohacking protocols” repository on Github in Markdown format with separate images. Enjoy! Life is, as ever these days, quite busy! I have two trips coming up, one to Ignite in Dublin and one to the Newcastle Maker Faire (where Brian Degger of Transitlab.org and I will be hosting a little workshop on a few DIYbio experiments and fun stuff).